王金
发布人：网站管理员  发布时间：2019-12-10   浏览次数:8084

  



王金 研究员

  

  

教育和职业经历:

09/2000-07/2004南京农业大学beat365在线登录app专业理学学士学位

09/2004-07/2009中科院上海beat365在线登录app研究院植物生理生态研究所微生物学博士学位

07/2009-07/2012香港中文大学微生物学系博士后

07/2012-11/2013中科院上海beat365在线登录app研究院植物生理生态研究所助理研究员

12/2013-04/2018中科院上海beat365在线登录app研究院植物生理生态研究所副研究员

05/2018-beat365在线登录app研究员（独立研究组）

  

个人研究方向：

主要研究方向是合成生物学使能技术的开发及其应用。近年来，申请人以第一作者或通讯作者身份在Cell Research、Nucleic Acids Research、ACS Synthetic Biology、Cell Discovery等杂志发表论文20多篇，并申请了多项发明专利。其中主要的成果包括：（1）开发了新型DNA体外无缝拼接/编辑技术，提高了大片段、复杂片段DNA的体外拼接/编辑效率和准确率。（2）提出了基于CRISPR/Cas12a的新型生物元器件拼接标准（C-Brick）。（3）开发了基于CRISPR的微生物基因编辑/基因调控技术，建成了不依赖于PAM位点的基因组编辑方法和基于CRISPR crRNA阵列的多基因高效转录调控的新方法。（4）开发了CRISPR新型分子诊断系统，该系统被Science杂志誉为下一代分子诊断技术。

发表论文(# first author; * corresponding author):

  1. Jin Wang and Guoping Zhao*. (2009) GlnR positively regulates nasA transcription in Streptomyces coelicolor.Biochemical and Biophysical Research Communications 386 (1): 77-81.

  2. Wei Zhao#, Yi Zhong#, Hua Yuan#, Jin Wang#, et al. (2010) Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism. Cell Research20(10):1096-1108.

  3. Ying Wang, XuFeng Cen, GuoPing Zhao* and Jin Wang* (2012) Characterization of a New GlnR Binding Box in the Promoter of amtB in Streptomyces coelicolor Inferred a PhoP/GlnR Competitive Binding Mechanism for Transcriptional Regulation of amtB. Journal of Bacteriology194(19): 5237-5244.

  4. WeiHua Chen, ZhongJun Qin, Jin Wang* and GuoPing Zhao* (2013) The MASTER (Methylation-Assisted Tailorable Ends Rational) Ligation method for seamless DNA assembly.Nucleic Acids Research41(8): e93.

  5. Ying Wang, JingZhi Wang, ZhiHui Shao, Hua Yuan, YinHua Lu, WeiHong Jiang, GuoPing Zhao*, Jin Wang* (2013) Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of the Amycolatopsis mediterranei nas Operon. Journal of Bacteriology 195(11): 2595-2602.

  6. Ying Wang#, Chen Li#, Na Duan#, Bing Li, XiaoMing Ding, YuFeng Yao, Jun Hu*, GuoPing Zhao*, Jin Wang*. (2014) GlnR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterranei U32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site. PLoS One 9(8): e104811.

  7. JiaKun Liu, WeiHua Chen, ShuangXi Ren, GuoPing Zhao*, Jin Wang*. (2014) iBrick: a new standard for iterative assembly of biological parts with homing endonucleases. PLoS One 9(10): e110852.

  8. Jin Wang*, Ying Wang, GuoPing Zhao (2015) Precise characterization of GlnR Box in actinomycetes. Biochemical and Biophysical Research Communications 458(3): 605-607.

  9. ZhuiHui Shao, ShuangXi Ren, XinQiang Liu, Jian Xu, Han Yan, GuoPing Zhao, Jin Wang* (2015) A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq. Microbial Cell Factories 14: 75.

  10. ZhiHui Shao#, WanXin Deng#, ShiYuan Li, JuanMei He, ShuangXi Ren, WeiRen Huang, YinHua Lu, GuoPing Zhao, ZhiMing Cai*, Jin Wang* (2015) GlnR-Mediated Regulation of ectABCD Transcription Expands the Role of the GlnR Regulon to Osmotic Stress Management. Journal of Bacteriology 197(19): 3041–3047.

  11. XuFeng Cen, JingZhi Wang, GuoPing Zhao, Ying Wang*, Jin Wang* (2016) Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor. Biochemical and Biophysical Research Communications 471(4):510-514.

  12. ShiYuan Li, GuoPing Zhao*, Jin Wang* (2016) C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1. ACS Synthetic Biology 5(12):1383-1388.

  13. Chao Lei#, ShiYuan Li#, JiaKun Liu#, Xuan Zheng, GuoPing Zhao, Jin Wang* (2017) The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Research45(9): e74.

  14. Chen Li#, XinQiang Liu#, Chao Lei, Han Yan, ZhiHui Shao, Ying Wang, GuoPing Zhao, Jin Wang*, XiaoMing Ding* (2017) RifZ (AMED_0655) is a pathway-specific regulator for rifamycin biosynthesis in Amycolatopsis mediterranei.Applied Environmental Microbiology 83(8): e03201-16

  15. Xuan Zheng#, ShiYuan Li#, GuoPing Zhao, Jin Wang* (2017) An efficient system for deletion of large DNA fragments in Escherichia colivia introduction of both Cas9 and the non-homologous end joining system from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications 485(4): 768-774

  16. ShiYuan Li, GuoPing Zhao, Jin Wang* (2017) Protocols for C-Brick DNA standard assembly using Cpf1.Journal of Visualized Experiments (124), e55775

  17. Hong Zhang, Qiuxiang Cheng, Aimin Liu*, Guoping Zhao, Jin Wang* (2017) A novel and efficient method for bacteria genome editing employing both CRISPR/Cas9 and an antibiotic resistance cassette. Frontiers in Microbiology 8: 812

  18. Xiaochun Zhang#, Jingman Wang#, Qiuxiang Cheng#, Xuan Zheng, Guoping Zhao, Jin Wang* (2017) Multiplex gene regulation by CRISPR–ddCpf1. Cell Discovery 3: 17018

  19. Chao Lei#, Jingzhi Wang#, Yuanyuan Liu, Xinqiang Liu, Guoping Zhao, Jin Wang* (2018) A feedback regulatory model for RifQ-mediated repression of rifamycin export in Amycolatopsismediterranei. Microbial Cell Factories17:14

  20. Shi-Yuan Li, Qiu-Xiang Cheng*, Jia-Kun Liu, Xiao-Qun Nie, Guo-Ping Zhao and Jin Wang* (2018) CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA. Cell Research 28: 491–493.

  21. Zhendong Li#, Xinqiang Liu#, Jingzhi Wang, Ying Wang, Guosong Zheng, Yinhua Lu, Guoping Zhao, Jin Wang* (2018) Insight into the molecular mechanism of the transcriptional regulation of amtB operon in Streptomyces coelicolor. Frontiers in Microbiology 9: 264

  22. Shi-Yuan Li#, Qiu-Xiang Cheng#, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guo-Ping Zhao and Jin Wang* (2018) CRISPR-Cas12a-assisted nucleic acid detection. Cell Discovery 4: 20.

  23. Shi-Yuan Li, Jia-Kun Liu, Guo-Ping Zhao and Jin Wang* (2018) CADS: CRISPR/Cas12a-Assisted DNA Steganography for Securing the Storage and Transfer of DNA-encoded Information. ACS Synthetic Biology 7(4):1174-1178.

  24. Yi Li#, Shi-Yuan Li#, Jin Wang*, Guo-Zhen Liu* (2018) CRISPR/Cas Systems towards Next-Generation Biosensing. Trends in Biotechnology 37(7): 730-743; DOI:https://doi.org/10.1016/j.tibtech.2018.12.005

  25. Jingman Wang#, Anrui Lu#, Jinxin Bei*, Weiren Huang, Zhiming Cai, Guoping Zhao*, Jin Wang* (2019) CRISPR/ddCas12a-based programmable and accurate gene regulation. Cell Discovery 5: 15.

  26. Anrui Lu#, Jingman Wang#, Weihong Sun*, Weiren Huang*, Zhiming Cai*, Guoping Zhao, Jin Wang* (2019) Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation. Cell Discovery 5: 22.

      

      

    E-mail:wangj01@hotmail.com